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Accumulation,Subcellular Distribution and Chemical Forms of Cadmium in Celery(Apium graveolens L.) Roots(PDF)


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Accumulation,Subcellular Distribution and Chemical Forms of Cadmium in Celery(Apium graveolens L.) Roots
ZOU YuanWANG XiaolongCHEN YaoyaoCHEN XuesuiXU Jie
(College of Biological,Chemical Sciences and Engineering,Jiaxing University,Jiaxing,Zhejiang 314001)
cadmium (Cd)celery (Apium graveolens L.)accumulationchemical formsubcellular distribution
A hydroponics experiment combined with subcellular fractionation and sequential extraction was conducted to study the Cd concentration in different organs of celery and the Cd subcellular distribution and chemical forms in the roots with different Cd doses in nutrient solution.The results showed that no apparent injuries could be observed in the aerial part of celery.However,root growth was restrained greatly under high Cd treatment.The roots demonstrated high accumulation capacity of Cd,the Cd subcellular distribution was in the order of soluble fraction>cell wall>organelle.In addition,the Cd content in both the soluble fraction(47.84 mg?kg-1 FW to the at most) and the cell wall (113.60 mg?kg-1FW to the at most) showed increasing trend with elevated Cd supply.Chemical forms of Cd in root changed with the Cd concentration as well.When the Cd concentration in solution was less than or equal to 20 mg?L-1,the NaCl-extractable Cd was the highest (32.45%-41.07%),which indicated that the combination of Cd with protein and pectic acid might play the dominant role;when Cd supply level was up to 40 mg?L-1,the C2H5OH-extractable Cd was the highest(62.30%),which suggested that because of the high concentration of Cd stress,the cell wall and the membrane permeability increased,so the Cd ions were active in the cell,the HCl-extractable Cd and HAc-extractable Cd were the lowest under all Cd treatments,which indicated that the content of insoluble phosphate and oxalic acid in celery root cell wall was supposed to be relatively lower,contributing to the limited immobilization of Cd.










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Last Update: 2017-03-22