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The Screening of Transgenic Plants Resistant to Downy Mildew Disease in Melon(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年01期
Page:
102-105
Research Field:
Publishing date:

Info

Title:
The Screening of Transgenic Plants Resistant to Downy Mildew Disease in Melon
Author(s):
NING Xue-feiHAN Xiao-luWANG Xian-leiGAO Xing-wangLI Guan
College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang 830046
Keywords:
melondowny mildewAt2?genetransgenic plants screening
PACS:
S 685.21
DOI:
-
Abstract:
Taking T1 melon of transgenic plant seed that transgened into At2downy mildew resistant gene though pollen tube pathway technology as material,swollen explants was gotten through tissue culture technology.Then PCR analysis was performed on the explant,which were obtained from transgenic seed to screen out positive transgenic plant rapidly.The result indicated that the optimal sterilization condition was 72℃ for 4 h and the optimal medium components with antifungal effect was MS salts+B5 vitamins+2 mg/L 6-BA+7 g/L agar +500 mg/L cefotaxime+1.1 g/L thiophanate-methyl,during the culture processes of melon explants.1 100 T1 generation seed explants transformed with downy mildew resistance genes?At2were detected using PCR technique,and the positive rate was 4.5 ‰.

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Last Update: 2014-08-08